2/19/2023 0 Comments Uvc medical abbreviation![]() 9– 12 These bacteria include methicillin-resistant Staphylococcus aureus (MRSA), coagulase-negative Staphylococcus, Streptococcus pyogenes, Enterococcus faecalis, Pseudomonas aeruginosa, and Mycobacteria. Several in vitro studies have reported the susceptibility of multidrug-resistant bacteria to inactivation by UVC. 8 With appropriate doses, UVC may selectively target microorganisms with a negligible effect on the mammalian cells. The UVC kills the organisms by damaging the DNA and RNA through dimerization of pyrimidine molecules. The ultraviolet C (UVC) light could be a potential alternative antimicrobial intervention to which resistance will be difficult to develop. 6, 7įailure of the antibiotics to manage biofilm-associated infections has led to a significant research effort to find alternative antimicrobial approaches with more efficacy and less resistance developed by the microorganisms. 5 Such resistance is demonstrated not only toward antibiotics but also toward preservatives, disinfectants, and antiseptics. They can be up to 1,000 times more resistant to antibiotics than their planktonic (free-floating) counterparts. The bacteria within the biofilm are protected from the host defense mechanisms and the antimicrobial agents. 3 The mortality associated with cardiovascular device-related, biofilm-associated infections, for example, is estimated to be 12%–25%, with a health care cost of $33,000–$35,000/event. Implant-associated infections lead to considerable morbidity, repeated surgeries, and prolonged antibiotic therapy. Since the microorganisms in such infections are in aggregates, they remain less susceptible to antimicrobial agents compared to single-cell suspensions tested in the diagnostic laboratories. 1 Microorganisms upon shedding from the biofilm enter the circulation and result in dissemination and establishment of infection at distant sites. In the medical setting, biofilm-associated infections constitute a steadily increasing problem and can start out from the surface of different indwelling devices. Keywords: alternative therapy, biofilm-associated infections, MRSA, MSSA, Staphylococcus epidermidis, indwelling medical devicesīiofilms are composed of pure or mixed communities of microorganisms adhering to surfaces. Further investigations are needed to assess the effectiveness of the combined approach in the clinical settings. The combined approach can be used as a therapeutic modality for managing biofilm-associated infections by preventing the establishment of biofilms and/or disrupting the formed biofilms on the inserted medical devices with the goal of increasing their usefulness and preventing infectious complications. Treatment of the biofilms with the antibiotics following their exposure to UVC light significantly ( P<0.001) reduced the number of viable cells within the biofilms but did not completely eradicate them.Ĭonclusion: To our knowledge, this combinatorial approach has not been investigated before. Results: Exposure of the bacterial biofilms to the UVC light or each of the antibiotics alone was ineffective in killing the bacteria. The effect of UVC radiation followed by treatment with an antistaphylococcal antibiotic on the viability of the bacteria in the biofilm was visualized using LIVE/DEAD BacLight bacterial viability stain and confocal laser scanning microscopy. The catheters were cut into segments and sonicated, and the number of the sessile cells was determined colorimetrically using XTT viable cells assay. Materials and methods: A novel biofilm device was used to assess the combined approach. The biofilms on the catheters were irradiated with the UVC light at 254 nm and irradiance of 6.4 mW followed by treatment with vancomycin or quinupristin/dalfopristin at twice their minimum bactericidal concentrations or with linezolid at 64 µg/mL for 24 hours. Objective: We evaluated the efficacy of sublethal dose of UVC light with anti-staphylococcal antibiotics against biofilms made from 30 isolates of methicillin-susceptible Staphylococcus aureus and methicillin-resistant S. The ultraviolet C (UVC) light is a potential therapy that can be used against the biofilm of bacterial pathogens. 1Department of Microbiology, Immunology and Biotechnology, Faculty of Pharmacy and Biotechnology, German University in Cairo, New Cairo City, Egypt 2Department of Internal Medicine, Division of Infectious Diseases, Eastern Virginia Medical School, Norfolk, VA, USAīackground: Biofilm formation inside inserted medical devices leads to their failure and acts as a source of refractory infections.
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